UJI TOKSISITAS AKUT EKSTRAK AIR DAUN BINAHONG (Andredera cordifolia(Ten.)Steenis) TERHADAP LARVA UDANG (Artemia salina Leach) DENGAN METODE BRINE SHRIMP LETHALLY TEST (BSLT)

Abstrak

Andredera leaves are potential medicinal plants that can overcome various types of diseases. The benefits of this plant are very large in the world of empirical medicine. This research was conducted with the aim to determine the level of toxicity of andredera leaf extract with extracts using water solvent (H₂O) and to find out at what concentrations Andredera leaf water extract can provide toxic effects. The research was carried out by extracting samples with a solvent of dried water using a freeze dryer. The obtained binahong water extract was used to test the toxicity of BSLT shrimp larvae with various concentrations of 200 ppm, 400 ppm, 600 ppm, 800 ppm, 1000 ppm, and 1200 ppm. Then the data of Artemia salina Leach's death was analyzed by probit analysis to determine the LC50 value.The results of the study showed that each concentration of Andredera leaf extract had a high level of toxicity to shrimp larvae Artemia salina Leach. Shown with an LC₅₀<1000 ppm value of 660,69 ppm. Based on the chemical content contained in Andredera leaves contained flavonoid, saponin, alkaloid, terpenoid in binahong leaves showed that these compounds showed a potential bioactivity against shrimp larvae Artemia salina Leach