Aktivitas Fraksi Daun Krinyuh (Eupatorium odoratum L.) Terhadap Bakteri Staphylococcus epidermis

Moh Rifal; Muhammad Aris; Hesty Setiawati

doi:10.47650/fito.v16i1.1528

Moh Rifal Universitas Pancasakti Makassar
Muhammad Aris Universitas Pancasakti Makassar
Hesty Setiawati Universitas Pancasakti Makassar

DOI: https://doi.org/10.47650/fito.v16i1.1528

Keywords: Daun Kirinyuh, Fraksinasi, Staphylococcus epidermidis

Abstract

Kirinyuh Leaf (Eupatorium odoratum L.) Contains Tannins, Phenols, Flavonoids, Saponins and Steroids. Flavonoids are known to have antibacterial activity. This study aims to determine the activity of kirinyuh leaf fraction against Staphylococcus epidermidis bacteria. Kirinyuh leaf extract was prepared by maceration method using 96% ethanol as solvent. The results obtained in the first observation are FI (7,33mm, 7,52mm, 7,35mm and average 7,4mm) FII (7,42mm, 7,56mm, 7,71mm and average 7,56mm) FIII (7,54mm7,59mm, 7,74mm and average 7,62mm) and FN (10,30mm, 11,13mm, 11,26mm and average 10,89mm) while in the second experiment, it was obtained FI (7,98mm, 8,07mm, 8,03mm and average8,02mm) FII (9,09mm, 8,78mm, 8,55mm and average 8,80mm) FIII (8,94mm, 8,83mm, 8,71mm and average 8,82mm) and FN (11,87mm, 12,13mm, 12,17mm and average 12,05mm). Kirinyuh leaves have antibacterial activity with an inhibitory power of at low concentrations with moderate inhibition (inhibition zone 5-10mm).

Daun kirinyuh (Eupatorium odoratum L.) mengandung tanin, fenol, flavonoid, saponin, dan steroid. Flavanoid diketahui memiliki aktivitas sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas fraksi daun kirinyuh (Eupatorium odoratum L.) terhadap bakteri Staphylococcus epidermidis. Ekstrak daun kirinyuh (Eupatorium odoratum L.) dibuat dengan metode maserasi dengan menggunakan pelarut etanol 96%. Hasil yang diperoleh pada pengamatan pertama yaitu FI (7,33mm, 7,52mm, 7,35mm dan rata-rata 7,4mm), FII (7,42mm, 7,56mm, 7,71mm dan rata-rata 7,56mm), FIII (7,54mm, 7,59mm, 7,74mm dan rata-rata 7,62mm) dan FN (10,30mm, 11,13mm, 11,26mm dan rata-rata 10,89mm). Sedangkan pada percobaan kedua diperoleh yaitu FI (7,98mm, 8,07mm, 8,03mm dan rata-rata 8,02mm), FII (9,09mm, 8,78mm, 8,55mm dan rata-rata 8,80mm), FIII (8,94mm, 8,83mm, 8,71mm dan rata-rata 8,82mm) dan FN (11,87mm, 12,13mm, 12,17mm dan rata-rata 12,05mm). Daun kirinyuh (Eupatorium odoratum L.) memiliki aktivitas sebagai antibakteri dengan daya hambat pada konsentrasi rendah dengan daya hambat yang sedang (zona hambat 5-10mm).