Uji Aktivitas Antibakteri Ekstrak Aseton Daun Teh Hijau (Camellia sinensis L.) Terhadap Isolat Salmonella enterica serovar Typhi Resisten

Abstract

Abstract: Handling and treatment of typhoid fever is becoming increasingly difficult because Salmonella typhi is found to be resistant to antibiotics, especially first line drugs such as Chloramphenicol, Amoxicillin, Tetarcycline and Cotrimoxazole. Research has been conducted on the Activity Test of Green Tea Leaf Acetone Extract (Camellia sinensis L.) against Salmonella enterica serovar Typhi Resistant isolates. This study aims to determine the antibacterial activity of Green Tea Leaf acetone extract against S. Typhi Resistant isolates. Green Tea leaves were extracted by maceration using acetone as a solvent. Initial sensitivity testing was carried out using paperdisk Chloramphenicol 30 μg, Tetracycline 30 μg, and Sulfamethoxazole-Trimethoprim 75 μg. The results of the sensitivity test showed that Chloramphenicol 30 μg and Tetracycline 30 μg were resistant while Sulfamethoxazole-Trimethoprim 75 μg were sensitive to S. Typhi resistance. Furthermore, the antibacterial inhibition test of Green Tea Leaf Extract with a concentration of 0.05% w/v; 0.75% w/v; 0.1 % w/v; 0.25 % w/v; 0.5 % w/v ; 0.75% w/v and 1% w/v in DMSO 10% w/v. The results showed that Green Tea Leaf extract showed a weak category of antibacterial inhibitory activity against Salmonella enterica serovar Typhi Resistant isolates.