Uji Aktivitas Antibakteri Fraksi Etil Asetat Daun Lamtoro (Leucaena leucocephala Lamk.) Terhadap Pertumbuhan Staphylococcus aureus Dan Escherichia coli
Abstract
This study aims to determine the antibacterial activity of the ethyl acetate fraction of Leucaena leucocephala leaves on the growth of Staphylococcus aureus and Escherichia coli and to determine at what concentration the ethyl acetate fraction of Leucaena leucocephala leaves has antibacterial activity against Staphylococcus aureus and Escherichia coli. The research design used was an experimental laboratory carried out at the Pharmaceutical Microbiology Laboratory, Pancasakti University, Makassar. Leucaena leucocephala leaves were extracted by maceration using 96% ethanol and then fractionated using a separating funnel using ethyl acetate solvent. Lamtoro leaf fractions were prepared with concentrations of 5 mg/ml, 10 mg/ml, and 15 mg/ml and Na-CMC as a negative control and Ciprofloxacin as a positive control. Antibacterial activity testing was carried out by the agar diffusion method using paper disks on NA medium with an incubation period of 1 x 24 hours and 2 x 24 hours at 370C. The results showed that the ethyl acetate fraction from Leucaena leucocephala leaves had antibacterial activity against the growth of Staphylococcus aureus and Escherichia coli. The ethyl acetate fraction of Leucaena leucocephala leaves at a concentration of 15 mg/ml gave the greatest inhibition, namely Staphylococcus aureus 13.84 mm while Escherichia coli 12.28 mm. The two way ANOVA statistical test showed that there was a significant difference between the antibacterial activity of Staphylococcus aureus and Escherichia coli with a sig value of p <0.05.
